Spectrofluorimetric Estimation of Scopoletin in Evolvulus alsinoides Linn. and Convulvulus pluricaulis Choisy.

A simple sensitive spectrofluorimetric method was developed for the analysis of total coumarins calculated as scopoletin in the plants Evolvulus alsinoides and Convulvulus pluricaulis. The fluorescent nature of scopoletin proved to be of immense value in the development of the spectrofluorimetric method. The excitation and emission wavelengths for scopoletin were 430 nm and 460 nm respectively and the instrument was Shimadzu RF 5301 PC spectrofluorophotometer. The method was validated in terms of linearity, accuracy and precision. The proposed spectrofluorimetric method provides a faster and cost effective qualitative and quantitative control for routine analysis of scopoletin in Evolvulus alsinoides and Convulvulus pluricaulis and their formulations.